Department of Chemistry

Laura Itzhaki Publications

2012

The how's and why's of protein folding intermediates.
Tsytlonok M, Itzhaki LS
Arch Biochem Biophys 2012, -. DOI: 10.1016/j.abb.2012.10.006

Using FlAsH to probe conformational changes in a large HEAT repeat protein.
Tsytlonok M, Itzhaki LS
Chembiochem 2012, 13, 1199-1205. DOI: 10.1002/cbic.201200012

Folding and binding: lingering questions, emerging answers.
Itzhaki LS, Rose GD
Curr Opin Struct Biol 2012, 22, 1-3. DOI: 10.1016/j.sbi.2012.01.001

Implications of 3D domain swapping for protein folding, misfolding and function
Rousseau F, Schymkowitz J, Itzhaki LS
Advances in Experimental Medicine and Biology 2012, 747, 137-152.

Implications of 3D domain swapping for protein folding, misfolding and function.
Rousseau F, Schymkowitz J, Itzhaki LS
Adv Exp Med Biol 2012, 747, 137-152. DOI: 10.1007/978-1-4614-3229-6_9

From artificial antibodies to nanosprings: the biophysical properties of repeat proteins.
Itzhaki LS, Lowe AR
Adv Exp Med Biol 2012, 747, 153-166. DOI: 10.1007/978-1-4614-3229-6_10

From artificial antibodies to nanosprings: The biophysical properties of repeat proteins
Itzhaki LS, Lowe AR
Advances in Experimental Medicine and Biology 2012, 747, 153-166.

2010

Toward Classification of BRCA1 Missense Variants Using a Biophysical Approach
Rowling PJE, Cook R, Itzhaki LS
J BIOL CHEM 2010, 285, 20080-20087. DOI: 10.1074/jbc.M109.088922

Nature and nurture in protein folding and binding (vol 20, pg 1, 2010)
Itzhaki L, Wolynes P
CURR OPIN STRUC BIOL 2010, 20, 397-397. DOI: 10.1016/j.sbi.2010.03.003

Characterising the binding specificities of the subunits associated with the KMT2/Set1 histone lysine methyltransferase.
Murton BL, Chin WL, Ponting CP, Itzhaki LS
Cell Cycle Proteins, Gene Expression Regulation, Enzymologic, Histone-Lysine N-Methyltransferase, Protein Subunits, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity 2010, 398, 481-488. DOI: 10.1016/j.jmb.2010.03.036

Mechanical unfolding of an ankyrin repeat protein.
Serquera D, Lee W, Settanni G, Marszalek PE, Paci E, Itzhaki LS
Ankyrin Repeat, Biophysics, Computer Simulation, Humans, Microscopy, Atomic Force, Muscle Proteins, Proteasome Endopeptidase Complex, Protein Conformation, Protein Denaturation, Protein Folding, Protein Kinases, Protein Structure, Tertiary, Proto-Oncogene Proteins, Signal Transduction, Stress, Mechanical, Time Factors 2010, 98, 1294-1301. DOI: 10.1016/j.bpj.2009.12.4287

Nature and nurture in protein folding and binding.
Itzhaki L, Wolynes P
Protein Binding, Protein Folding, Proteins 2010, 20, 1-2. DOI: 10.1016/j.sbi.2010.01.007

Erratum to 'Nature and nurture in protein folding and binding' [Curr Opin Struct Biol 2010, 20:1-2]
Itzhaki L, Wolynes P
Current Opinion in Structural Biology 2010, 20, 397-397.

2009

Alcohol oxidase (AOX1) from Pichia pastoris is a novel inhibitor of prion propagation and a potential ATPase
Zhang H, Loovers HM, Xu LQ, Wang MZ, Rowling PJE, Itzhaki LS, Gong WM, Zhou JM, Jones GW, Perrett S
MOL MICROBIOL 2009, 71, 702-716. DOI: 10.1111/j.1365-2958.2008.06557.x

2008

Shifting transition states in the unfolding of a large ankyrin repeat protein
Werbeck ND, Rowling PJE, Chellamuthu VR, Itzhaki LS
P NATL ACAD SCI USA 2008, 12, 9982-9987. DOI: 10.1073/pnas.0705300105

The quest to understand protein folding
Itzhaki LS, Wolynes PG
CURR OPIN STRUC BIOL 2008, 1, 1-3. DOI: 10.1016/i.sbi.2008.01.001

The quest to understand protein folding
Itzhaki LS, Wolynes PG
CURR OPIN STRUC BIOL 2008, 1, 1-3. DOI: 10.1016/i.sbi.2008.01.001

Shifting transition states in the unfolding of a large ankyrin repeat protein (Proceedings of the National Academy of Sciences of the United States of America (2008) 105, (9982-9987) DOI: 10.1073/pnas.0705300105)
Werbeck ND, Rowling PJE, Chellamuthu VR, Itzhaki LS
Proceedings of the National Academy of Sciences of the United States of America 2008, 0, 13696-13696.

2007

ATPase site architecture and helicase mechanism of an archaeal MCM.
Moreau MJ, McGeoch AT, Lowe AR, Itzhaki LS, Bell SD
Adenosine Triphosphate, Archaeal Proteins, Binding Sites, Computer Simulation, DNA Helicases, Hydrolysis, Metalloendopeptidases, Models, Chemical, Models, Molecular, Monte Carlo Method, Multiprotein Complexes, Mutagenesis, Site-Directed, Protein Conformation, Protein Subunits, Sulfolobus solfataricus 2007, 41, 304-314. DOI: 10.1016/j.molcel.2007.08.013

Probing a moving target with a plastic unfolding intermediate of an ankyrin-repeat protein.
Werbeck ND, Itzhaki LS
Ankyrin Repeat, Circular Dichroism, Humans, Protein Engineering, Protein Folding, Protein Structure, Tertiary 2007, 24, 7863-7868. DOI: 10.1073/pnas.0610315104

Hsp40 interacts directly with the native state of the yeast prion protein Ure2 and inhibits formation of amyloid-like fibrils
Lian HY, Zhang H, Zhang ZR, Loovers HM, Jones GW, Rowling PJE, Itzhaki LS, Zhou JM, Perrett S
J BIOL CHEM 2007, 33, 11931-11940. DOI: 10.1074/jbc.M606856200

Rational redesign of the folding pathway of a modular protein.
Lowe AR, Itzhaki LS
Ankyrin Repeat, Intercellular Signaling Peptides and Proteins, Kinetics, Models, Molecular, Mutant Proteins, Mutation, Protein Conformation, Protein Folding 2007, 35, 2679-2684. DOI: 10.1073/pnas.0604653104

Biophysical characterisation of the small ankyrin repeat protein myotrophin.
Lowe AR, Itzhaki LS
Animals, Ankyrin Repeat, Biophysics, Intercellular Signaling Peptides and Proteins, Kinetics, Magnetic Resonance Spectroscopy, Models, Molecular, Models, Statistical, Mutation, Proline, Protein Conformation, Protein Folding, Rats, Thermodynamics, Urea 2007, 20, 1245-1255. DOI: 10.1016/j.jmb.2006.10.060

2006

Activation of ubiquitin ligase SCF(Skp2) by Cks1: insights from hydrogen exchange mass spectrometry.
Yao ZP, Zhou M, Kelly SE, Seeliger MA, Robinson CV, Itzhaki LS
CDC2-CDC28 Kinases, Carrier Proteins, Cyclin-Dependent Kinases, Deuterium, Enzyme Activation, Humans, Hydrogen, Mass Spectrometry, Models, Molecular, Peptides, Protein Conformation, S-Phase Kinase-Associated Proteins, SKP Cullin F-Box Protein Ligases, Solvents 2006, 363, 673-686. DOI: 10.1016/j.jmb.2006.08.032

Domain swapping in p13suc1 results in formation of native-like, cytotoxic aggregates.
Rousseau F, Wilkinson H, Villanueva J, Serrano L, Schymkowitz JW, Itzhaki LS
Animals, Cell Cycle Proteins, Deuterium, Humans, Hydrogen, Models, Molecular, Protein Conformation, Protein Folding, Protein Renaturation, Protein Structure, Tertiary, Schizosaccharomyces pombe Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization 2006, 363, 496-505. DOI: 10.1016/j.jmb.2006.07.061

Folding and fibril formation of the cell cycle protein Cks1
Bader R, Seeliger MA, Kelly SE, Ilag LL, Meersman F, Limones A, Luisi BF, Dobson CM, Itzhaki LS
AMYLOID-LIKE FIBRILS, CRYSTAL-STRUCTURE, NEURODEGENERATIVE DISEASES, HUNTINGTONS-DISEASE, GLUTAMINE REPEATS, EXPANDED GLUTAMINE, PROLINE RESIDUES, CORE STRUCTURE, BETA-HELIX, IN-VITRO 2006, 281, 18816-18824. DOI: 10.1074/jbc.M603628200

2005

Role of conformational heterogeneity in domain swapping and adapter function of the Cks proteins.
Seeliger MA, Spichty M, Kelly SE, Bycroft M, Freund SM, Karplus M, Itzhaki LS
J Biol Chem 2005, 280, 30448-30459. DOI: 10.1074/jbc.M501450200

Crystal structure of the ENT domain of human EMSY.
Chavali GB, Ekblad CM, Basu BP, Brissett NC, Veprintsev D, Hughes-Davies L, Kouzarides T, Itzhaki LS, Doherty AJ
J Mol Biol 2005, 350, 964-973. DOI: 10.1016/j.jmb.2005.05.047

Binding of EMSY to HP1beta: implications for recruitment of HP1beta and BS69.
Ekblad CM, Chavali GB, Basu BP, Freund SM, Veprintsev D, Hughes-Davies L, Kouzarides T, Doherty AJ, Itzhaki LS
EMBO Rep 2005, 6, 675-680. DOI: 10.1038/sj.embor.7400415

2004

Investigation of protein interations between Cks1 and Skp2 using hydrogen exchange nano-electrospray mass spectrometry
Yao Z, Seeliger MA, Itzhaki LS, Robinson CV
PROTEIN SCIENCE 2004, 0, 86-86.

Cooperative organization in a macromolecular complex (vol 10, pg 718, 2003)
Seeliger MA, Breward SE, Friedler A, Schon O, Itzhaki LS
NAT STRUCT MOL BIOL 2004, 0, 678-678.

Comparison of BRCT domains of BRCA1 and 53BP1: a biophysical analysis.
Ekblad CM, Friedler A, Veprintsev D, Weinberg RL, Itzhaki LS
Amino Acid Sequence, BRCA1 Protein, Binding Sites, Calorimetry, Carrier Proteins, Chromatography, Gel, Cysteine Endopeptidases, Electrophoresis, Polyacrylamide Gel, Humans, Intracellular Signaling Peptides and Proteins, Models, Molecular, Molecular Sequence Data, Peptide Fragments, Phosphoproteins, Protein Binding, Protein Denaturation, Protein Folding, Protein Structure, Tertiary, Recombinant Proteins, Spectrometry, Fluorescence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Thermodynamics, Tumor Suppressor Protein p53, Ultracentrifugation 2004, 13, 617-625. DOI: 10.1110/ps.03461404

Intermediates control domain swapping during folding of p13suc1.
Rousseau F, Schymkowitz JW, Wilkinson HR, Itzhaki LS
Binding Sites, Cell Cycle Proteins, Chromatography, Gel, Dimerization, Kinetics, Mutagenesis, Protein Folding, Protein Structure, Secondary, Recombinant Proteins, Schizosaccharomyces pombe Proteins, Thermodynamics 2004, 14, 8368-8377. DOI: 10.1074/jbc.M310640200

Erratum: Cooperative organization in a macromolecular complex (Nature Structural and Molecular Biology (2003) 10 (718-724))
Seeliger MA, Breward SE, Friedler A, Schon O, Itzhaki LS
Nature Structural and Molecular Biology 2004, 678-678.

2003

Cooperative organization in a macromolecular complex.
Seeliger MA, Breward SE, Friedler A, Schon O, Itzhaki LS
Adaptor Proteins, Signal Transducing, Amino Acids, Anisotropy, Binding Sites, Calorimetry, Cell Cycle Proteins, Cyclin-Dependent Kinase Inhibitor p27, Dose-Response Relationship, Drug, Kinetics, Macromolecular Substances, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Models, Molecular, Mutation, Phosphopeptides, Protein Binding, Protein Structure, Tertiary, S-Phase Kinase-Associated Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Spectrometry, Fluorescence, Structure-Activity Relationship, Thermodynamics, Tumor Suppressor Proteins 2003, 19, 718-724. DOI: 10.1038/nsb962

The unfolding story of three-dimensional domain swapping.
Rousseau F, Schymkowitz JW, Itzhaki LS
Animals, Humans, Protein Structure, Tertiary, Proteins, Thermodynamics 2003, 90, 243-251.

Weak cooperativity in the core causes a switch in folding mechanism between two proteins of the cks family.
Seeliger MA, Breward SE, Itzhaki LS
Amino Acid Sequence, CDC2-CDC28 Kinases, Carrier Proteins, Cell Cycle Proteins, Cyclin-Dependent Kinases, Humans, Kinetics, Models, Molecular, Mutagenesis, Site-Directed, Mutation, Protein Binding, Protein Conformation, Protein Denaturation, Protein Folding, Protein Kinases, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Structure-Activity Relationship, Thermodynamics 2003, 16, 189-199.

Sequential unfolding of ankyrin repeats in tumor suppressor p16.
Tang KS, Fersht AR, Itzhaki LS
Ankyrin Repeat, Cyclin-Dependent Kinase Inhibitor p16, Humans, Models, Molecular, Mutation, Protein Denaturation, Protein Folding, Protein Structure, Secondary, Protein Structure, Tertiary, Urea 2003, 48, 67-73.

2002

Three different binding sites of Cks1 are required for p27-ubiquitin ligation
Sitry D, Seeliger MA, Ko TK, Ganoth D, Breward SE, Itzhaki LS, Pagano M, Hershko A
J BIOL CHEM 2002, 277, 42233-42240. DOI: 10.1074/jbc.M205254200

Characterisation of the BRCT domains of the breast cancer susceptibility gene product BRCA1.
Ekblad CM, Wilkinson HR, Schymkowitz JW, Rousseau F, Freund SM, Itzhaki LS
J Mol Biol 2002, 320, 431-442.

The structure of the transition state for folding of domain-swapped dimeric p13suc1.
Rousseau F, Schymkowitz JW, Wilkinson HR, Itzhaki LS
Structure 2002, 10, 649-657.

Folding and association of the human cell cycle regulatory proteins ckshs1 and ckshs2.
Seeliger MA, Schymkowitz JW, Rousseau F, Wilkinson HR, Itzhaki LS
Biochemistry 2002, 41, 1202-1210.

2001

Observation of signal transduction in three-dimensional domain swapping.
Schymkowitz JW, Rousseau F, Wilkinson HR, Friedler A, Itzhaki LS
Nat Struct Biol 2001, 8, 888-892. DOI: 10.1038/nsb1001-888

Three-dimensional domain swapping in p13suc1 occurs in the unfolded state and is controlled by conserved proline residues.
Rousseau F, Schymkowitz JW, Wilkinson HR, Itzhaki LS
Proc Natl Acad Sci U S A 2001, 98, 5596-5601. DOI: 10.1073/pnas.101542098

2000

Sequence conservation provides the best prediction of the role of proline residues in p13suc1.
Schymkowitz JW, Rousseau F, Itzhaki LS
J Mol Biol 2000, 301, 199-204. DOI: 10.1006/jmbi.2000.3958

The folding pathway of the cell-cycle regulatory protein p13suc1: clues for the mechanism of domain swapping.
Schymkowitz JW, Rousseau F, Irvine LR, Itzhaki LS
Structure 2000, 8, 89-100.

1999

Stability and folding of the tumour suppressor protein p16.
Tang KS, Guralnick BJ, Wang WK, Fersht AR, Itzhaki LS
J Mol Biol 1999, 285, 1869-1886. DOI: 10.1006/jmbi.1998.2420

1998

Stability and folding of the cell cycle regulatory protein, p13(suc1).
Rousseau F, Schymkowitz JW, Sánchez del Pino M, Itzhaki LS
J Mol Biol 1998, 284, 503-519. DOI: 10.1006/jmbi.1998.2173

A reply to Englander and Woodward
Clarke J, Itzhaki LS, Fersht AR
TRENDS BIOCHEM SCI 1998, 23, 379-381.

Synergy between simulation and experiment in describing the energy landscape of protein folding.
Ladurner AG, Itzhaki LS, Daggett V, Fersht AR
Proc Natl Acad Sci U S A 1998, 95, 8473-8478.

Hydrogen exchange and protein folding.
Clarke J, Itzhaki LS
Curr Opin Struct Biol 1998, 8, 112-118.

1997

Complementation of peptide fragments of the single domain protein chymotrypsin inhibitor 2.
Ladurner AG, Itzhaki LS, de Prat Gay G, Fersht AR
J Mol Biol 1997, 273, 317-329. DOI: 10.1006/jmbi.1997.1303

Hydrogen exchange at equilibrium: a short cut for analysing protein-folding pathways?
Clarke J, Itzhaki LS, Fersht AR
Trends Biochem Sci 1997, 22, 284-287.

Hydrogen exchange at equilibrium: a short cut for analysing protein-folding pathways?
Clarke J, Itzhaki LS, Fersht AR
Trends Biochem Sci 1997, 22, 284-287.

Hydrogen exchange in chymotrypsin inhibitor 2 probed by mutagenesis.
Neira JL, Itzhaki LS, Otzen DE, Davis B, Fersht AR
J Mol Biol 1997, 270, 99-110. DOI: 10.1006/jmbi.1997.1088

Hydrogen exchange in chymotrypsin inhibitor 2 probed by denaturants and temperature.
Itzhaki LS, Neira JL, Fersht AR
J Mol Biol 1997, 270, 89-98. DOI: 10.1006/jmbi.1997.1049

Following co-operative formation of secondary and tertiary structure in a single protein module.
Neira JL, Itzhaki LS, Ladurner AG, Davis B, de Prat Gay G, Fersht AR
J Mol Biol 1997, 268, 185-197. DOI: 10.1006/jmbi.1997.0932

Strain in the transition state of folding and in the native state of proteins
Ladurner A, Itzhaki L, Fersht A
PROTEIN ENG 1997, 10, 29-29.

Strain in the folding nucleus of chymotrypsin inhibitor 2.
Ladurner AG, Itzhaki LS, Fersht AR
Fold Des 1997, 2, 363-368.

1996

Structure of the transition state for folding of a protein derived from experiment and simulation.
Daggett V, Li A, Itzhaki LS, Otzen DE, Fersht AR
J Mol Biol 1996, 257, 430-440. DOI: 10.1006/jmbi.1996.0173

Solvent isotope effects on the refolding kinetics of hen egg-white lysozyme.
Itzhaki LS, Evans PA
Protein Sci 1996, 5, 140-146. DOI: 10.1002/pro.5560050117

1995

The structure of the transition state for folding of chymotrypsin inhibitor 2 analysed by protein engineering methods: evidence for a nucleation-condensation mechanism for protein folding.
Itzhaki LS, Otzen DE, Fersht AR
J Mol Biol 1995, 254, 260-288. DOI: 10.1006/jmbi.1995.0616

Search for nucleation sites in smaller fragments of chymotrypsin inhibitor 2.
Itzhaki LS, Neira JL, Ruiz-Sanz J, de Prat Gay G, Fersht AR
J Mol Biol 1995, 254, 289-304. DOI: 10.1006/jmbi.1995.0617

Nature and consequences of GroEL-protein interactions.
Itzhaki LS, Otzen DE, Fersht AR
Biochemistry 1995, 34, 14581-14587.

Movement of the position of the transition state in protein folding.
Matouschek A, Otzen DE, Itzhaki LS, Jackson SE, Fersht AR
Biochemistry 1995, 34, 13656-13662.

Folding of a nascent polypeptide chain in vitro: cooperative formation of structure in a protein module.
De Prat Gay G, Ruiz-Sanz J, Neira JL, Itzhaki LS, Fersht AR
Proc Natl Acad Sci U S A 1995, 92, 3683-3686.

1994

Single versus parallel pathways of protein folding and fractional formation of structure in the transition state.
Fersht AR, Itzhaki LS, elMasry NF, Matthews JM, Otzen DE
Proc Natl Acad Sci U S A 1994, 91, 10426-10429.

Structure of the transition state for the folding/unfolding of the barley chymotrypsin inhibitor 2 and its implications for mechanisms of protein folding.
Otzen DE, Itzhaki LS, elMasry NF, Jackson SE, Fersht AR
Proc Natl Acad Sci U S A 1994, 91, 10422-10425.

Tertiary interactions in the folding pathway of hen lysozyme: kinetic studies using fluorescent probes.
Itzhaki LS, Evans PA, Dobson CM, Radford SE
Biochemistry 1994, 33, 5212-5220.

1990

A unique binding cavity for divalent cations in the DNA-metal-chromomycin A3 complex.
Itzhaki L, Weinberger S, Livnah N, Berman E
Biopolymers 1990, 29, 481-489. DOI: 10.1002/bip.360290303